Abstract CRISPR-associated (Cas) transposases (CAST) are RNA-guided systems capable of programmable integration of large segments of DNA without creating double-strand breaks. Engineered Cascade CAST function in human cells but are challenging to deploy due to the complexity of the targeting components. Unlike Cascade. which require three Cas proteins. https://www.roneverhart.com/Rag-Bone-Dress-Marylebone-Black-Leather-Silk-Chiffon-Size-2-Tiered-Mini-NEW/